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  • Article title
  • Abstract
  • Keywords
  • Introduction
  • Aim
  • Materials and methods
    • Tissue fixation
    • RNA extraction methods
    • Method A: lysis buffer
    • Method B: lysis buffer and phenol based extraction
    • Method C: phenol-based extraction
    • DNase treatment
    • cDNA synthesis
    • cDNA preamplification
    • Quantitative Real-Time PCR (qPCR)
    • Standard curve
    • Statistical analysis
  • Results
    • RNA yield and purity
    • Quantitative PCR
    • Preamplification efficacy assessment
  • Discussion
  • Conclusions
  • Acknowledgements
  • References
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