Original Article |
Corresponding author: Stavroula Baka ( sbaka@aretaieio.uoa.gr ) © 2022 Kalliopi Anagnostopoulou, Despina Tzanakaki, Alexandros Gryparis, Stella Demeridou, Stavroula Baka.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Anagnostopoulou K, Tzanakaki D, Gryparis A, Demeridou S, Baka S (2022) Seminal plasma visfatin levels negatively correlate with sperm concentration. Folia Medica 64(2): 283-287. https://doi.org/10.3897/folmed.64.e61657
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Introduction: Visfatin is involved in nicotinamide adenine dinucleotide biosynthesis, with a possible role in spermatogenesis. We investigated seminal plasma visfatin levels and its possible correlations with sperm parameters (concentration, motility, morphology) and BMI.
Materials and methods: We included 79 semen samples obtained from men from infertile couples presenting for sperm analysis. The samples were divided into 2 groups: a group of 35 samples with normal sperm parameters and another group of 44 samples with at least one abnormal sperm parameter. Seminal plasma visfatin levels were determined using commercially available enzyme-linked immunosorbent assay kits.
Results: Demographic data and body mass index (BMI) were similar in our subjects. As expected, the sperm parameters were significantly different between the 2 groups we studied. Visfatin levels did not differ between groups (66.6 ng/ml in normal samples and 72.7 ng/ml in abnormal samples, p=0.114) and did not correlate with sperm motility, sperm morphology, and BMI. However, a negative correlation between visfatin levels and sperm concentration (r=−0.28; p=0.014) and sperm count (r=−0.3; p=0.009), respectively, was detected.
Conclusions: Visfatin was detected in all human seminal plasma samples. Although its levels were similar in subjects with and without normal sperm parameters, a role for visfatin in sperm physiology cannot be ruled out at this point and further research is required.
infertility, semen parameters, seminal plasma, visfatin
Infertility has increased worldwide over the past few decades and the medical community is always in search of possible causes and better therapeutic management. Since infertility has been linked to obesity, the adipokines, which are secreted by the adipose tissue, could represent the link between obesity and infertility. In contrast to female infertility, where possible relationships to different adipokines have been extensively investigated, the male counterpart has been less studied.[
Visfatin, also known as nicotinamide phosphoribosyltransferase (NAMPT), is an adipocytokine with potential role in different metabolic and immune disorders.[
Ocόn-Grove et al.[
Interestingly, although visfatin expression was demonstrated in a variety of tissues including testes[
Thus, our objective was to detect seminal plasma visfatin levels and to investigate the association, if any, between visfatin and the sperm parameters.
Our sample comprised 79 men from infertile couples presenting for sperm analysis at our hospital. The sample was divided into two groups: 35 with normal sperm parameters, and 44 with at least one abnormal sperm parameter. The study was approved by the Ethics Committee of our teaching hospital, and informed consent was obtained from each participant.
Sperm analysis was performed manually according to the WHO criteria.[
For the statistical analysis, normality was examined by Kolmogorov-Smirnov test. Data regarding seminal plasma visfatin were not normally distributed; therefore, non-parametric procedures (Wilcoxon signed rank test) were used. Variables not normally distributed are presented as median (range). Spearman correlation coefficient was applied to detect any positive or negative correlations between visfatin and other variables, while multiple regression analysis was performed to examine the effect of different confounding factors on visfatin. P values less than 0.05 were considered statistically significant, while values ≥0.05 ≤0.10 were considered as indicative. Statistical analysis was performed using SPSS v. 17.0 (Chicago, IL, USA).
Demographic data, sperm analysis results, and visfatin levels in the study population are presented in Table
By means of Wilcoxon test, no significant correlation was detected between visfatin and any of the sperm parameters except for normal morphology where only an indicative correlation was observed (p=0.084).
Using Spearman correlation coefficient, visfatin levels were significantly negatively correlated with sperm count (r=−0.3; p=0.009) and sperm concentration (r=−0.28, p=0.014).
Multiple regression analysis revealed only sperm count (p=0.021) and sperm concentration (p=0.011) as confounding factors.
Demographic and biochemical data in subjects with normal and abnormal sperm parameters
Variables | Total samples | Normal samples | Abnormal samples | P |
Valid N | 79 | 35 | 44 | |
Age (years) | 40 (28-52) | 40 (28-51) | 40 (33-52) | 0.420 |
Weight (kg) | 85 (57-140) | 82 (62-139) | 89 (57-140) | 0.161 |
Height (cm) | 170 (165-194) | 170 (165-190) | 180 (170-194) | 0.055 |
BMI (kg/m2) | 27.4 (19.3-47.0) | 26.8 (19.4-47.0) | 28.1 (19.3-43.2) | 0.646 |
Sperm volume (ml) | 3.4 (1.0-8.1) | 3.6 (1.6-6.3) | 3.1 (1.0-8.1) | 0.140 |
Sperm concentration (×106/ml) | 30 (<0.1-190) | 57 (15-190) | 10 (<0.1-170) | <0.001 |
Sperm count (×106) | 85.0 (<0.1-858.6) | 224.0 (52.5-858.6) | 35.5 (<0.1-231.0) | <0.001 |
Total motility (%) | 58 (0-90) | 69 (37-90) | 51 (0-82) | <0.001 |
Normal morphology (%) | 6 (1-19) | 8 (4-19) | 3 (1-19) | <0.001 |
Visfatin (ng/mL) | 71.3 (8.1-442.4) | 66.6 (8.1-96.5) | 72.7 (10.0-442.4) | 0.114 |
In the last decades, it has been proven that white adipose tissue is more than a fat storing organ capable of secreting significant proteins that regulate metabolic homeostasis such as the adipokines. The purpose of this study was to detect visfatin, one of the adipokines, in the seminal plasma of men from infertile couples presenting for sperm analysis, and correlate its levels with the investigated semen parameters.
Sperm analysis is usually the first diagnostic test carried out for infertility assessment and, as a result, multiple sperm parameters are investigated to assess sperm quality as well as probable fertilization potential.[
In agreement with previous data[
BMI and visfatin levels did not differ between the groups under investigation. Previous work demonstrated the association between increased BMI and abnormal sperm parameters, in particular increased prevalence of azoospermia or oligozoospermia.[
Visfatin is an important enzyme for energy metabolism and apoptosis, with regulatory effect on NAD, a co-substrate for dehydrogenases which have been implicated in spermatogenesis. Ocόn-Grove et al.[
Our study has some limitations. As adipose tissue is an important factor in relation to the visfatin levels, its effect could not be investigated since our groups did not differ regarding their BMI. Sperm analysis did not include some other parameters such as DNA fragmentation test or ROS levels, which might have offered important correlations. We presented only statistical relationships and speculations as for the role visfatin might play in male fertility, and did not present any proof for the physiologic effects on the spermatozoa. Although it has been demonstrated that adipokines have a significant role in different physiological processes, and some of them specifically on male reproduction, there are still many aspects that need to be elucidated. In this direction, experimental studies could shed light on the specific mechanisms visfatin uses to influence sperm function, if any.
We demonstrated that visfatin was present in seminal plasma and was negatively correlated with sperm concentration and sperm count in men from infertile couples. Since a definitive functional role in human male reproduction has not yet been attributed to visfatin, the mechanisms that govern its role in male fertility must be revealed through further experiments and studies.