Case Report |
Corresponding author: Hasan Burnusuzov ( hasan.burnusuzov@mu-plovdiv.bg ) © 2023 Hasan Burnusuzov, Ivan Yankov, Kostadin Ketev, Mariana Murdjeva.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Burnusuzov H, Yankov I, Ketev K, Murdjeva M (2023) Primary immunodeficiency screening in an infant with cytomegalovirus disease reveals HIV infection. Folia Medica 65(1): 166-170. https://doi.org/10.3897/folmed.65.e72203
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Cytomegalovirus is widely spread worldwide, and it is not uncommon for it to complicate the congenital human immunodeficiency virus (HIV) disease as an acquired or congenital coinfection. However, the association of the two infections is not common amongst infants with primary immune deficiencies.
We describe a case of a 6-month-old infant with acquired cytomegalovirus and HIV infections, diagnosed in the course of the patient’s clinical and laboratory workup for a presumed primary immunodeficiency. To date, this is the first reported case of such a combination in a child from Bulgaria.
congenital immune disorder, children, flow cytometric workup, complex viral illness
Cytomegalovirus (CMV) is the commonest congenital infection, affecting approximately 0.6%-0.7% of all newborns in the developing countries.[
A 6-month-old boy was brought to our hospital by his grandmother for a second time, with fever (up to 38°C), mild cough, and transient macular rash. The previous hospitalization was a month before for otitis. The family history, according to the grandmother, was unremarkable. Past history: born abroad, after an uneventful pregnancy and normal delivery on term, with small-for-date anthropometric measurements: birth length 48 cm and birth weight 2400 g. The child was fed formula milk since birth, but with poor weight gain. At the age of three months, he was admitted to a hospital in Germany because of vomiting, failure to thrive, and chest X-ray proven interstitial pneumonia. Then an acquired CMV infection was detected by RT-PCR CMV-replication tests in blood and urine and negatives such in the stored neonatal screening heel-prick filter paper. After 15 days of antibiotic and supportive treatment, the patient still did not improve and the parents decided to leave the hospital, neglecting doctors’ warnings.
Physical examination: alert, but in poor general condition with moderate dehydration signs and normal capillary refill. A generalized non-itchy erythema-macular rash was seen. There was bilateral coarse vesicular breathing. The liver was palpated at 3.5 cm below the costal margin, soft and non-tender, and the spleen was at 1 cm below the costal margin, with similar characteristics. The rest of the physical exam was normal for the age. Anthropometrics: weight – 4900 g (below the 3rd percentile), height – 61.5 cm (the 3rd percentile), head circumference – 38.5 cm (below the 3rd percentile).
Complete blood count: HGB: 102 g/L; RBC: 3.9×1012/L; HCT: 0.33; MCV: 80.1 fl; PLT: 640×109/L; WBC: 25.24×109/L; Reticulocytes: 1.11%; ESR: 50 mm/h. Table
Tests | % | Reference range (%) | Absolute count (×109/l) | Reference range (×109/l) |
Neutrophils | 15.6 | 22-45 | 3.93 | 2.2-4.5 |
Eosinophils | 1.3 | 0-4.1 | 0.32 | 0-0.6 |
Basophils | 1.3 | 0-2 | 0.32 | 0-0.1 |
Monocytes | 5.8 | 5.8-11.8 | 1.47 | 0.4-0.8 |
Lymphocytes | 69.7 | 35-74 | 17.6 | 3.5-7.3 |
Test | Result | Reference range |
Total protein, g/l | 77.1 | 57-80 |
Albumin, g/l | 41.0 | 35-55 |
Total bilirubin, mcmol/l | 13.3 | 3.4-21 |
Direct bilirubin, mcmol/l | 5.6 | 0.8-8.5 |
CRP, mg/l | 17.5 | 0-10 |
AST, U/l | 456.0 | 0-60 |
ALT, U/l | 502.0 | 0-45 |
LDH, U/l | 862.0 | 230-975 |
GGT, U/l | 129.0 | 0-55 |
Alkaline phosphatase, U/l | 307.0 | 42-362 |
Urea, mmol/l | 4.6 | 3.2-8.2 |
Creatinine, mcmol/l | 32.0 | 35-75 |
Glucose, mmol/l | 5.3 | 2.8-6.1 |
Fibrinogen, g/l | 2.91 | 2.0-4.5 |
aPTT, seconds | 28.1 | 24-35 |
PT, % | 109.8 | 70-120 |
IgG, g/l | 14.4 | 3.72-12.7 |
IgM, g/l | 1.492 | 0.35-2.42 |
IgA, g/l | 0.319 | 0.0-0.83 |
IgE, IU/ml | 35.3 | 0-12.7 |
Flow cytometric lymphocyte subpopulations, TBNK-panel results (T, B lymphocytes and NK cells)*
Tests | % | Reference range % | Absolute count (×109/l) | Reference range (×109/l) |
Lymphocytes | 17.025 | 3.8-9.9 | ||
CD3+ T cells | 58 | 50-77 | 9.837 | 2.4-6.9 |
CD3+CD4+T helper cells | 11 | 33-58 | 1.803 | 1.4-5.1 |
CD3+CD8+T cytotoxic/suppressor cells | 39 | 13-26 | 6.596 | 0.6-2.2 |
CD19+ B cells | 34.9 | 13-35 | 5.945 | 0.7-2.5 |
CD3-CD16+CD56+NK cells | 6.7 | 2-13 | 1.134 | 0.1-1.0 |
CD4/CD8 index | - | - | 0.27 | 1.6-3.8 |
Blood gas analysis and urine tests – within reference range. Chest X-ray: increased bilateral vascularity. Upper-left sided infiltrative opacities. Mild hilar lymphadenopathy. Normal-sized heart. No effusions. Abdominal ultrasound: hepatosplenomegaly, diffuse homogenous hyperechoic liver structure. Normoechoic spleen. No ascites. Normal kidneys. No abdominal lymphadenopathy. Virology studies: serology – positive for anti-CMV IgG – 182.5 RU/ml (reference range <22 RU/ml); Anti-CMV IgM – 1.47 RU/ml (reference range <1.1 RU/ml); positive for anti-HIV1/2 Ag/Ab – ELISA and Western blot test. Negative anti-SARS-CoV-2 by PCR and antigen tests. TST (Mantoux test) <5 mm and T-Spot-TB- negative.
Treatment and disease course: proper rehydration and broad-spectrum antibiotic therapy led to improvement after two days of high fever and the rash waned, but the weight gain was poor yet. Thus, a comprehensive immune deficiency directed workup was initiated. High serum IgG, elevated CD19+B-lymphocytes and prominent CD4 lymphopenia with inverted CD4/CD8 index from the TBNK-panel were found. Consequently, HIV infection was proven. Finally, during the discussion about the HIV-testing, the parents reluctantly reported that the father had been HIV-positive for 12 years and on antiretroviral therapy (ART), but the mother had never been tested, nor had she received any ART. Accordingly, an antiretroviral therapy was started.
We describe the process of an immune deficiency screening in an infant with CMV infection. Traditionally, the serum anti-CMV IgM and IgG levels of the baby and the mother are analyzed in order to qualify the infection as congenital or acquired, together with quantitative RT-PCR for CMV replication. In unclear situations, the molecular testing of both the blood and the Guthrie card of the infant is proposed as a useful diagnostic method for accurate differentiation between congenital and acquired CMV infection, which was utilized in our case.[
We can only speculate about the HIV status of the mother and the way of the HIV and CMV acquisition by the infant. Of note, it was born small for date and did not grow normally, despite the adequate care. ART is reported to decrease the rates of pre- and perinatal HIV transmission, so is the formula feeding of infants born to HIV positive mothers, although a horizontal transmission is also documented, which might be the case in our patient.[
The social sensitivity and stigmatization about HIV, especially in small societies or ethnic groups, are well documented.[
The reported case is a diagnostic challenge, further complicated by ethical issues and the overlapping clinical presentations of HIV and CMV in an infant. In any child with symptomatic CMV infection, primary or secondary immune deficiency must be considered. We acknowledge that testing for HIV should always be performed in the initial workup, then followed up by the more elaborate and expensive immunological tests. It is also appropriate to use the blood and the Guthrie card PCR-method for rapid differentiation of congenital from acquired CMV infection in the early infancy.